NucleoCounter NC-3000

Advanced Imaging Cytometer

Chemometec NucleoCounter NC-3000

Main features

  • Variety of tests
  • Simple to Use
  • Cell analysis cycle in 5 minutes
  • 1-Minute Apoptosis Assay
  • 1 minute GFP assay
  • Advanced High-Speed Cell Counting
  • Open platform with 5 channels
Chemo metec
Contador celular automatizado

Design and Usability

The NucleoCounter ® NC-3000™ is a state-of-the-art cytometer that uses fluorescence imaging to characterize cell properties.

The NC-3000™ can perform cell counting and viability determination at high speed and includes plug-and-play analytical assays for mammalian, yeast and insect cells. Predefined assays include a single 5-minute cell cycle test with a corresponding selection of apoptosis assays.

With the FlexiCyte™ license, the user can create protocols by choosing the light source, emission filters and detailed settings. The accompanying intuitive software can be used for data analysis of the results and has unlimited licenses.

The NucleoCounter® NC-3000™ advanced imaging cytometer ensures flow analysis with high reproducibility and no calibration required..

Main characteristics:

  • No rinsing or clogging
  • Easy to operate – no warm-up or calibration
  • CFR 21 Part 11: Ready
  • Automated PDF reports
  • Unlimited software licenses and free software updates
  • Excellent field application specialist support
  • High-speed cell counting and advanced cell analysis
  • Visual inspection of cells – tracing to image
  • Images are automatically saved and can be reanalyzed later
  • Low sample volume
  • Build your own assays with up to 4 analytical channels
  • Data export to FCS Express, FlowJo, etc.

NucleoCounter® NC-3000™ Cell Cytometry Analysis in the Lab

Advanced Imaging Cytometer

More features:

The scientist can count the cells manually, visually verifying the count. This verification can also be performed with the intuitive NucleoView™ software included with the NC-3000™. Scatter plots and images are interrelated, allowing visual verification of cell populations.

NucleoView™ software allows easy coupling between the image obtained and the graphs showing fluorescence for accurate determination of the events to be counted.

NucleoView plot NC 3000

The closed cell counting system, which uses self-contained disposable cassettes, helps the user to be protected and to be when handling samples. The absence of pipettes, the exposure to (toxic) reagents and minimal cleaning make the NucleoCounter® a safe choice. NucleoCounter® a safe choice.

can be toxicity

The NucleoCounter® NC-3000™ can count cells even from a sample of highly aggregated cells or cells growing in clusters.

In the Aggregate Cell Assay, the total cell count is obtained from a lysed aliquot of the sample (see schematic below).

Lysis disrupts the cell membrane, dismantles the clumps and releases the suspended nuclei. Nuclei are stained with DAPI and counted automatically. Since dead cells are usually less abundant and tend to detach from the aggregates, the dead cell count is determined from the unlysed aliquot (figure below).

The combination of these two measurements results in high precision and accuracy even for the most difficult groups.

Conteo de ce%CC%81lulas en grupos con NucleoCounter%C2%AE NC 200%E2%84%A2

Cell cycle analysis is the fundamental method for establishing abnormalities in cell division and, therefore, symptoms of cell damage. With ChemoMetec’s unique 2-step cell cycle, a cycle can be performed in 5 minutes including cell treatment.

Its profile will be displayed in the accompanying PlotManager software and the percentage or number of events in G0/G1, S-phase, G2 and sub-G1 can be easily identified.

Cell Cycle Analysis of Jurkat cells

With the NucleoCounter® NC-3000™ plug-and-play GFP transfection assay, the number of viable and dead cells transfected with GFP can be determined with high accuracy.

GFP transfected cells with mock 1024x752 1

The NucleoCounter-3000™ is ideal for regulated quality control, production and even R&D and PD departments. The instrument and accompanying software can be easily integrated into existing computer networks.

NC 3000 Black with laptop

The FlexiCyte™ module enables users to perform advanced cellular analysis of a wide range of biomarkers and fluorescent proteins in mammalian cells.

FlexiCyte NC 3000 1

Easy and accurate determination of viability and count of primary mesenchymal stem cells derived from lipoaspirates and bone marrow aspirates.

Counting of Mesenchymal Stem Cells

Chemometec NucleoCounter NC-3000

Advanced Imaging Cytometer

Specifications table.

Product Number 970-3002 (Ready Analytical Assays)

970-3003 (FlexiCyte™)

Optics Lens with x2 magnification, 1/2″ CCD with 1392 x 1040 pixels
Sampling Device
Via1-Cassette™
, NC-Slide A2™ and NC-Slide A8™.
Excitation (nm) Seven LED light sources c at 3on peaks at 65, 405, 475, 530, 630 nm + 2 white LEDs
Emission (nm) Nine interchangeable emission filters: 430/20, 470/55, 475/15, 530/15, 560/35, 580/25,

675/75, 630 LP, 740/60

Sample Consumption Approx. 60 μl (cassettes), Approx. 30 μl (A2 slides), Approx. 10 μl (slides A8)
Analyzed Volume 3.2 μl sample (cassettes), 0.8-16 μl sample (slide chamber)
Optimum range 5 x 104 – – 5 x 106 cells/ml (for counting)
Type of cells Mammary cells, yeast cells, insect cells, avian cells
Minimum resources Windows 7, 8 or 10, USB 2.0, screen resolution 1366 x 768 pixels,

4 GB RAM and 10 GB free disk space

Data Presentation Images, tables, histograms, scatter plots, scatter plots
Weight/Dimensions 14 kg (30.9 lb); 29 x 29 x 31 cm (29 x 29 x 31 cm) [Alto x Ancho x Profundidad]
Feeding 100-240V~ 50-60Hz
Consumption 5/50W (in power save/peak mode)

LED emission filters schema NC 3000

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